b'Unstained protein ladders Unstained protein ladders are useful when you need tothus is an apparent molecular weight. Unstained protein accurately determine the size of your protein. However,ladders do not have this issue because the migration of the proteins can only be visualized after staining withthe proteins in the ladder are not affected by added dye, a Coomassie stain or other nonspecific protein stain.and thus migrate as normal (although one can observe The dyes in prestained protein ladders add additionaldifferences from one gel chemistry to the next), and as molecular weight to the ladder proteins and thus affectsuch are suitable for the most accurate determination of their migration. Each dye molecule may not bind equallymolecular weights in a protein sample. to a given protein or proteins, and some prestained protein ladders utilize multiple different types of coloredThese ready-to-use ladders include proteins of known dyes (for easier tracking of specific proteins in the ladder).molecular weight. Visualize them on SDS-PAGE gels Because of the dyes in prestained protein ladders, aby staining with Coomassie Brilliant Blue R250 or after given ladder proteins true molecular weight is altered andwestern transfer using Ponceau S or a similar stain. Low range Broad range Broad range kDa kDa200150120100 100857030 6025 502015 4010 3053.4 252015Gel 10 BlotNovex 816% Tris-glycine16% Tricine Gel gel (SDS-PAGE) Thermo Scientific PageRuler UnstainedThermo Scientific PageRuler Unstained Thermo Scientific PageRuler Unstained Low Range Protein Ladder Protein Ladder Broad Range Protein LadderCat. No. 26632 Cat. No. 26614 Cat. No. 26630High range High range Native kDa kDa kDa1,2361,0481,236250 1,048 720200 480720150 242120 480100 14685 242 66416% gel70 146312% gel60 662020NuPAGE3-8% Tris-Acetate Gel NativePAGE Bis-Tris Gel;stained with Coomassie R-250 dyeThermo Scientific PageRuler UnstainedInvitrogen HiMark Unstained Invitrogen NativeMark Unstained High Range Protein Ladder Protein Standard Protein StandardCat. No. 26637 Cat. No. LC5688 Cat. No. LC072550'