b'ProtocolsDI H 2 O S DI H 2 O DI H 2 O1. Wash gel 3 times 2. Add SimplyBlue3. Wash gel with 4. Additional wa ter wash with ultrapure DI water. SafeStain.100 mL of DI water. with 100 mL of DI water for increased sensitivity.5 minutes 1 hour 1 hour 1 hourFigure 32. SimplyBlue SafeStain protocol.DI H 2 O S DI H 2 O1. Wash gel 3 times2. Add Imperial Protein Stain.3. Water destain.with DI water. 15 minutes 5 minutes1 hour 15 minutesovernightFigure 33. Imperial Protein Stain protocol.DI H 2 O S DI H 2 O DI H 2 O1. Wash gel 3 times 2. Add PageBlue Protein3. Rinse gel 2 times 4. Wash gel 1 timewith ultrapure DI water. Staining Solution.with DI water. with DI water.30 minutes 1 hour 1 minute 5 minutesFigure 34. PageBlue Protein Staining Solution protocol.65'