b'1. 2. 3. Place the cell lid on the unit Drop buffer core into the lowerLock the gel tension wedge in buffer chamber of the XCellplace, load samples, and filland youre ready to run.SureLock Mini-Cell. Insert onethe buffer chambers with the mini gel in front of the bufferappropriate running buffers.core and a second mini gel or the buffer dam behind the buffer core.Figure 27. How to use the XCell SureLock Mini-Cell.NuPAGE Bis-Tris gel inXCell SureLock Mini-CellFigure 28. Electrophoresis of NuPAGE Bis-Tris gel with the XCell SureLock Mini-Cell. Lane 1: SeeBlue Plus2 Prestained Standard; lane 2:10 g E. coli lysate; lane 3: Mark12 Unstained Standard (blend of 12 purified proteins); lane 4: 40 g HeLa cell lysate; lane 5: 20 g HeLa cell lysate; lane 6: not used; lane 7: 40 g Jurkat cell lysate; lane 8: 5 g of a GST fusion protein; lane 9: Novex Sharp Unstained Protein Standard; lane 10: 5 g -galactosidase. Gel electrophoresis was performed at 200 V (constant), and gels were stained using SimplyBlue SafeStain. Image was acquired using a flatbed scanner.Recommended productsThe XCell SureLock Mini-Cell can be easily adapted for transfer of proteins from mini gels to membranes by simply inserting the Invitrogen XCell II Blot Module into the lower buffer chamber. 57'