b'Table 1. Key considerations for selecting the right bioconjugation reagent.Chemical reactivityFirst, select a reagent with the functional group(s) to bind your biomolecules of interest.NHS ester reaction Maleimide reactionO O O ONH 2 + O N pH 7 O + N SH + R N pH 6.57.5 R N SR O R N HOO H O O OAmine-containing NHS ester Amine bond NHS Sulfhydryl-containing Maleimide Thioether bondmolecule compound molecule compoundMolecular propertiesSecond, choose which features or characteristics are important for your application.Functionality Cleavability Structural modificationsO OO O O O N O FN O O N N O S S O N O FO O O HO O O N O O FO 4 FDSSsame reactivity DSP with disulfide linker for cleavage DBCOcopper-free click moiety on both endsSpacer arm composition Spacer arm length Spacer arm structureO O OO O ON N N N O OO HO O O NH 2O O O nOBMH with hydrocarbon spacer AMAS 4.4 short spacer between CA(PEG) n adds solubility inreactive groups aqueous solutionsApplicationsSelect the specific reagent depending on the application (e.g., protein detection, immobilization, or interaction studies).Conjugation Immobilization Protein interaction studiesO OO O O O ONa+O - O N O N N O S O NN O O N O O OO S O OO O O O OOSulfo-SMCC DSScouples proteins to surfaces DSSOMS-cleavable crosslinkerPackaging optionsSelect a package size or grade based on the scale of your reaction or your requirements. These reagents are available from milligram to kilogram quantities.Milligram Milligram to gram Milligram to gram Gram to kilogramBulk-sizepackagesavailableSingle tubesCatalog product Premium-grade Large-volume or custom packagesProtein bioconjugation, crosslinking, and modification thermofisher.com/proteinbiology 103'