b'Tools to optimize your cloning stepGeneArt Gene Synthesis and GeneOptimizer softwareThe power of custom gene synthesis is the ability to design your DNA without the constraints of traditional cloning. Equally important to most researchers, however, is obtaining high yields of mRNA and, ultimately, protein from synthetic genes. We developed Invitrogen GeneArt GeneOptimizer software to maximize the expression of synthetic genes in all commonly used expression systems.Tuning the expression level by choosing the optimal promoter and terminator combination can be an essential part of your expression project. The origin of replication also has a significant influence on the expression level of the foreign protein. We offer a broad range of commercially available, predesigned vectors optimized for various expression systems.Learn more at thermofisher.com/genesynthesisGibson Assembly kits and GeneArt Strings DNA Fragments Invitrogen GeneArt Gibson Assembly cloning kits provide highly efficient, seamless cloning, enabling the assembly of multiple DNA fragments of varying length into any vector.When combined with Invitrogen GeneArt Strings DNA Fragments orInvitrogen GeneArt Gene Synthesis, these cloning kits can be used to build simpleconstructs as well as large and demanding constructs from multiple fragments.Learn more about GeneArt Gibson Assembly cloning kits for protein expression at thermofisher.com/gibsonassemblyGateway cloningInvitrogen Gateway cloning technology is rapid, robust, and suited for high-throughput clone generation for protein production. It allows shuffling between different expression systems in just a few simple steps. Choose from a diverse selection of host systems, including E. coli, yeasts, and insect or mammalian cells, each of which utilizes unique destination vectors for all your expression applications. Additionally, the Invitrogen Gateway Vector Conversion System can convert specialized or customized vectors into an Invitrogen Gateway destination vector to suit your expression workflow.Select the right Gateway vectors for your workflow and review our cloning protocols at thermofisher.com/gatewayOne Shot chemically competent E. coliE. coli cells are widely used for production of recombinant proteins quickly, economically, and on a large scale. The most popular strain for recombinant protein expression is BL21 and its derivatives. Invitrogen One Shot chemically competent cells, such as BL21 Star (DE3) and BL21-AI cells, are optimized for high-level protein or toxic protein expression from T7 promoters. All are induced with IPTG or L-arabinose and come in the convenient Invitrogen One Shot format, allowing for transformation and recovery in a single tube.Choose chemically competent cells optimized for protein expression at thermofisher.com/compcellsCloning and protein expression thermofisher.com/proteinbiology 7'