b'Immunoassay platforms Biomarker quantitation assay ELISA is a plate-based assay technique designed for detectingplatformsand quantifying substances such as peptides, proteins,Highly referenced kits you can trustantibodies, and hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with anTo facilitate the investigation of inflammatory and other relevant antibody that is linked to an enzyme. Detection is accomplishedbiological markers, the Invitrogen portfolio has a broad menu by assessing the conjugated enzyme activity via incubationof immunoassays to easily detect and quantify proteins. Options with a substrate to produce a measurable product. The mostare available for single- and multi-biomarker quantitation assays, crucial element of the detection strategy is a highly specificas well as the appropriate instrument platforms for each type antibodyantigen interaction. of assay, so you can choose the platform you need to publish with confidence.ELISAs are typically performed in 96-well (or 384-well) polystyrene plates, which will passively bind antibodies and proteins. It is thisTailored to meet your needsbinding and immobilization of reagents that makes ELISAs soA variety of assay platforms are available including plate- and easy to design and perform. Having the reactants of the ELISAbead-based solutions across many different species. ELISA immobilized to the microplate surface makes it easy to separatekit formats include complete, ready-to-use kits, as well as bound from unbound material during the assay. This ability topreoptimized reagents to make your own. In addition, a variety wash away nonspecifically bound materials makes the ELISAof singleplex and multiplex panels for protein quantitation with a powerful tool for measuring specific analytes within a crudeInvitrogen ProcartaPlex assays are available. See Table 1 for preparation. a performance comparison of our immunoassay platforms and A detection enzyme or other tag can be linked directly to theTable 2 for a component list.primary antibody or introduced through a secondary antibody thatInvitrogen coated ELISA kitsquantitate with confidence. recognizes the primary antibody. It also can be linked to a proteinHighly verified ELISA kits with precoated plates provide lower such as streptavidin if the primary antibody is biotin labeled. Theinter- and intra-assay variability with ready-to-use reagents most used enzyme labels are horseradish peroxidase (HRP) andthat help ensure consistent data.alkaline phosphatase (AP). Other enzymes have been used asInvitrogen Instant ELISA kitsthe one-wash Instant well, but they have not gained widespread acceptance becauseELISA kit introduces fewer handling steps and requires of limited substrate options. A large selection of substrates islower hands-on time to increase productivity.available for performing the ELISA with an HRP or AP conjugate.Invitrogen phosphospecific ELISA kitsmeasure The choice of substrate depends upon the required assayphosphospecific proteins in cell lysates.sensitivity and the instrumentation available for signal detection (spectrophotometer, fluorometer, or luminometer). Invitrogen antibody pair kitskeep costs low with our affordable coat-it-yourself ELISA plate sets. Each set Though not as sensitive as fluorescent or chemiluminescentcontains the reagents required to prepare and run the substrates, chromogenic substrates are used most frequently;ELISA, including ELISA-optimized matched antibody pairs, they allow direct visualization and enable kinetic studies to bestandards, detection reagents, coating buffers, sample diluent, performed. Furthermore, chromogenic ELISA substrates areand tetramethylbenzidine (TMB) substrate solution. Plates are optional.detected with standard absorbance plate readers common to many laboratories.Invitrogen ProQuantum high-sensitivity (HS) assay kitstake advantage of a platform innovation that provides Invitrogen ProcartaPlex multiplex immunoassays are basedresearchers with an easy-to-run, high-performance assay, on Luminex xMAP technology, a bead-based multiplexingutilizing proximity-based amplification technology to combine technology in which beads are internally labeled with fluorescentthe analyte specificity of high-affinity antibodyantigen binding dyes to produce a specific spectral address. Instead of attachingwith the signal detection and amplification capabilities of real-time PCR to achieve a highly sensitive protein quantitation capture antibodies to a plate surface, capture antibodies areassay, using very small sample volumes.conjugated to the surface of beads. This technology uses flow cytometric or imaging methods for identification of the beads asProcartaPlex multiplex immunoassaysquantitate more well as detection of the bound antigens by the associated reporterwith less sample. ProcartaPlex multiplex immunoassays utilize Luminex xMAP technology for profiling up to 80 analytes in a molecules labeled with different dyes. The Luminex technologysingle 2550 L sample.enables multiple proteins to be detected simultaneously in each well of a 96-well plate, using a very small sample volume.74 Immunoassays thermofisher.com/proteinbiology'