b'iBlot 2 Gel Transfer DeviceThe Invitrogen iBlot 2 Gel Transfer Device is for dry electroblotting of proteins from mini-, midi-, and Invitrogen E-PAGE gels onto nitrocellulose or PVDF membranes for western blotting. The iBlot 2 system offers exceptional transfer efficiency, convenience, and speed, producing crisp and clear bands that remain sharp and straight (Figure 2). Buffer ion reservoirs incorporated into the gel matrix (transfer stacks as opposed to buffer tanks or soaked filter papers) enable rapid protein transfer to either nitrocellulose or PVDF membranes. The shortened distance between electrodes, along with high field strength and current, reduces run times to 7 minutes. With the iBlot 2 system, there is no need to prepare buffers, pretreat your gel, presoak filter papers, or clean up after blotting.Highlights: A B CRapid transferengineered for protein transfer in just 7 minutesMinimal preparation and cleanuptransfer stacks streamline transfer setup and teardownConvenienttouchscreen interface, preprogrammed andBolt Bis-Tris Plus,NuPAGE Bis-Tris,Novex Tris-glycine, optimized transfer protocols, and prepackaged ready-to-useNC NC NCstacks for transferring midi and mini blots (1 midi blot or 2 miniD E Fblots at a time)View the how-to video: Bolt Bis-Tris Plus,NuPAGE Bis-Tris,Novex Tris-glycine, PVDF PVDF PVDFFigure 2. Membranes processed on the iBlot 2 Gel Transfer Device show consistent transfer among various protein gel compositions to both nitrocellulose (NC) and PVDF membranes. Total cell extracts from A431 cells were transferred from Invitrogen 412% Bolt, 412% NuPAGE, and 420% Tris-glycine precast gels to NC membranes (AC) and to PVDF membranes (DF).Learn more at thermofisher.com/iblot2For more information, or to view additional products, go to thermofisher.com/proteintransferWestern blotting thermofisher.com/proteinbiology 63'