b'Recombinant protein expression systemsAchieving optimal and reliable amountsTable 2. Expression system selection guide.of recombinant protein is easier because of our wide selection of trusted mammalian, insect, yeast, bacterial,Expression and cell-free protein expressionsystem Human (HEK293) Hamster (CHO) Insect (Sf9, Sf21) Yeast Bacterial Cell-freeFeaturedMembrane proteins (GPCRs, ion channels,Ig-related proteins Intracellular proteins Industrial enzymes Bacterial proteins Rapid protein expressionsystems (Table 2). Backed by a team ofapplication other membrane proteins)experienced professionals to help youOther Difficult-to-express proteinsMembrane proteinsToxic proteins Low-complexity proteins Low-complexity proteinsToxic proteinsquickly optimize your protein expressionapplicationsSecreted proteinsSecreted proteinsMulti-protein complexesIn vitro labelingexperiments, we can accelerate yourPosttranslationalFull Nearly full Partial Partial Limited Partialresearch and development by offering: modificationsProtein yield Up to 1 g/L Up to 3 g/L Up to 900 mg/L Up to 10 g/L Up to 10 mg/L Up to 750 mg/LHigher protein yields (320x higher than other systems) RecommendedExpi293 expression system kits ExpiCHO Expression System Kit ExpiSf Expression SystemEasySelect Pichia Expression Kit Champion pET SUMO Expression System Cell-free in vitrohost system kit Starter Kit pBAD TOPO TA Expression KitFaster protein productionExpi293F Cells ExpiCHO-S Cells (cGMP Banked) ExpiSf9 Cells PichiaPink Expression Strain Set One Shot BL21 Star (DE3) Chemically HeLa and CHO extracts(days vs. weeks or months) RecommendedExpi293F GnTI Cells ExpiCHO-S Cells GS115, Pichia pastoris Yeast Strain Competent E. coli cellscells Expi293F Inducible Cells X-33, Pichia pastoris Yeast Strain BL21-AI One Shot Chemically High cell density Expi293F Inducible GnTI Cells Competent E. coli cellsLower cost per mg of protein RecommendedExpi293 Expression Medium ExpiCHO Expression Medium ExpiSf CD Medium PichiaPink Media Kit MagicMedia E. coli Expression Medium None requiredmedia YPD Broth LB BrothComplete, optimized systems ofRecommendedExpiFectamine 293 Transfection Kit ExpiFectamine CHO Transfection Kit ExpiFectamine Sf TransfectionNeon Transfection System Starter Pack None required None requiredcells, media, transfection reagents,transfectionReagentenhancers, feeds, and vectors and deliveryRecommendedpcDNA3.4 TOPO vector pcDNA3.4 TOPO vector pFastBac 1 vector pPINK (LC and HC) Champion pET SUMO vector T7 cell-free expression vectorsexpressionpFastBac Dual Expression Vector pPICZ A, B, and C Pichia Vectors pBAD TOPO vectorThe Gibco Expi platforms are examplesvectors pPICZ A, B, and C Pichia Vectors pRSET bacterial expression vectorsof expression systems that are fast andNalgene shake flasks Nalgene shake flasks Nalgene shake flasks Nalgene shake flasks Nalgene shake flasksRecommended cost-effective with high yield. Thesecultureware Nunc bioreactor tubes Nunc bioreactor tubes Nunc bioreactor tubessystems combine the advantages ofNunc plates Nunc plates Nunc platesRecommendedM-PER Mammalian ProteinM-PER Mammalian ProteinI-PER Insect Cell ProteinY-PER Yeast Protein Extraction Reagent B-PER Complete Bacterial Protein None requiredboth transient and stable strategiesextractionExtraction Reagent Extraction Reagent Extraction Reagent Extraction Reagentfor high expression of diverse proteinsreagent kitExpi293 Met () Protein Labeling Kit(Figure 3). For example, the GibcoRecommended13 protein labeling L-Methionine (Methyl- C)Expi293Expression System usesL-SelenomethionineHEK293 cells and can yield up to 1 g/L ofExpi293 Expression Medium ExpiCHO Stable Production Medium ExpiSf CD Medium Bacto CD Supreme Fermentationprotein in just 57 days. Single-use bioreactor vessels EfficientFeed C+ 2X Supplement Single-use bioreactor vessels Production Medium (FPM)Scale-up Efficient-Pro Feed 2 Single-use bioreactor vessels20.0 Single-use bioreactor vessels18.0 Services option Gene-to-protein services Gene-to-protein services Gene-to-protein services16.014.0Foldyieldincrease 12.0 Figure 3. External collaborator results. In collaboration with 22 labs, expression levels of 98 different proteins were tested using the Expi293 10.0 Expression System. The following results were obtained:8.0 87% of proteins demonstrated increased expression in the Expi293 system compared with the users current system6.0 4.6x average increase for all proteins (n = 98)4.0 4.0x average increase for mAbs (n = 54); highest level was 826 mg/L2.0 5.3x average increase for non-mAbs (n = 44); highest level was 790 mg/L0.08 Cloning and protein expression thermofisher.com/thermofisher.com/proteinbiology'