b'Western blottingWestern blotting was introduced in the late 1970s and is now a routine and fundamental technique for protein analysis. Western blotting, also called protein blotting or immunoblotting, uses antibodies to identify specific protein targets after they have been separated by electrophoresis and transferred to a membrane. The specificity of the antibodyantigen interaction enables a target protein to be detected in a complex protein mixture, such as cell or tissue lysate. Western blotting can be used to generate qualitative and semi-quantitative data for a protein of interest.Wet transfer systems60 Protein biology workowRapid transfer systems62Cloning andproteinWestern blot detection64 expressionCrudeOrdering information72 proteinProtein Protein Proteinsample purication gelpreparation electrophoresisImmunoassaysWesternblottingProtein bioconjugation, Mass Cryo-EM samplecrosslinking, spectrometry preparationand modicationWestern blotting thermofisher.com/proteinbiology 57'