b'Protein modificationProtein analysis and detection techniques often require more than direct conjugation with a bifunctional crosslinker or activated labeling reagent. For example, in many situations, specialized protein modifications are needed to add molecular mass, increase solubility for storage, or create a new functional group that can be targeted in a subsequent reaction step. Our extensive portfolio of modification reagents enables you to modify your protein for your specific experimental need (Table 3).Highlights:Convenientready-to-use reagents and convenient single-use packaging availableBroad portfolioextensive offering of reagents to increase solubility, block or derivatize functional groups, and reduce proteinsHigh qualityproducts manufactured in ISO 9001 certified facilitiesTable 3. Overview of key protein modification reagents.Reagent Reactive groups Application2-Mercaptoethanol Thiol Reducing agentSodiumCyanoborohydride Reduce Schiff base to alkylamine linkagecyanoborohydrideCA(PEG) n Amine Reversibly block primary amines (CA(PEG)); PEGylation of protein or surface, terminating Carboxylic acid with a carboxylic acid or primary amineCitrionic anhydride NA Reversibly block primary aminesCT(PEG) Carboxylic acid PEGylation of gold or metal surfaces, terminating with a carboxylic acidThiolCysteine HCl Thiol Reducing agentDTT, Clelands reagent Thiol Reducing agentEthylenediamine 2HCl AmineAddition of amines to protein or surfaceGuanidine HCl NA Protein denaturantHydroxylamine NA Deprotecting SATA-modified moleculesIodoacetic acid Iodoacetyl S-carboxymethylation of sulfhydryls (reduced cysteines)MA(PEG) n Amine PEGylation of a protein, oxidized carbohydrate, or surface terminating with a methyl groupMM(PEG) 12 , MM(PEG) 24 Maleimide Branched PEGylation of protein or surface, terminating with methyl groupMS(PEG) n N-hydroxysuccinimide ester PEGylation of a protein or surface, terminating with methyl groupNEM Maleimide Irreversible blocking of sulfhydryl groupsSATA N-hydroxysuccinimide ester Modification of primary amines with protected sulfhydrylSodium meta-periodate Periodate Oxidize carbohydrates for reductive aminationSodium NHS-acetate N-hydroxysuccinimide ester Irreversibly block aminesTCEP Phosphine Reducing agentTrauts reagent Iminothiolane Modify primary amines to contain a free sulfhydryl groupUrea NA Reducing agentLearn more at thermofisher.com/proteinmodificationProtein bioconjugation, crosslinking, and modification thermofisher.com/proteinbiology 105'