b'Table 3. Imaging modes available on iBright Imaging Systems.Imaging mode What examples of signal can be captured?Colorimetric signals from stains like Thermo Scientific Pierce Silver Stain or Invitrogen SimplyBlue Protein gels SafeStain (gels) and Thermo Scientific Pierce Reversible Protein Stain or Ponceau S (membranes); fluorescent signals from stains like Invitrogen SYPRO Ruby Protein Gel Stain (gels).Signals from ethidium bromide and a variety of fluorescent nucleic acid stains like Invitrogen Nucleic acid gels SYBR stains.Chemiluminescence with horseradish peroxidase (HRP) and alkaline phosphatase (AP) substrates like Chemi blots Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates. Fluorescence from visible and near-infrared (NIR) fluorophores like Invitrogen AlexaFluor and Fluorescent blots AlexaFluor Plus conjugates.Custom mode to image with multiple signals (chemiluminescent, fluorescent, colorimetric, and/or Universalvisible signals). Image display is similar to display in fluorescent blot mode and allows false colors to be assigned to any sample.Core imaging applications Specialty platebased imaging applicationsFluorescentChemiluminescentColorimetricallyCombinedVisible coloniesFluorescent western blots western blots stained westernfluorescent and(e.g., stained withcolonies (e.g.,blots chemiluminescentcrystal violet dye)GFP expression)western blotsQualitative visible imaging* applicationsFluorescentlyFluorescentlyColorimetricallyColorimetricallyLeaf sections Thin layer stained nucleicstained protein gelsstained protein gels stained membranes chromatography acid gels (TLC) platesFigure 6. Examples of imaging applications. The data were captured in grayscale. Pseudocolor (false color) can be applied for visualization purposes.* Enables qualitative visualization of samples and signal confirmation. Not recommended for quantitation.View the overview video:Test your protein research knowledge Question: Which detection method is most suitable for the detection of more than one antigen on a western blot (i.e., multiplex compatibility)?A. Colorimetric B. Chemiluminescent C. FluorescentCapture your data faster and more easily than ever before. Learn more at thermofisher.com/ibright Answer: CWestern blotting thermofisher.com/proteinbiology 71'