b'Cryo-EM sample preparation productsVitroEase Cryo-EM Training Kit and Apoferritin StandardThe Thermo Scientific VitroEase Cryo-EM Training Kit and Apoferritin Standard provide a complete solution for the successful preparation of grids for cryo-EM SPA.Highlights:Positive control samplehighly pure apoferritin protein verified for use in cryo-EM grid preparation and analysisHigh-qualitygrids and accessories from trusted sourcesConvenientready-to-use protein standard with step-by-step instructions for successful grid preparationThe VitroEase Cryo-EM Training Kit has been designed to provide the necessary components to get started with cryo-EM analysis of protein samples. The kit includes a positive control protein and commonly used accessories used in grid preparation, including tweezers for manipulation, grids, and grid boxes for storage. Detailed step-by-step instructions guide the user through the lengthy vitrification process. The VitroEase Apoferritin Standard has been designed specifically to meet the stringent needs for high protein purity, minimal protein aggregation, and intact protein structure. Apoferritin, a 24-subunit protein (474 kDa), is considered the gold standard for cryo-EM SPA (Figure 2). The standard is available in the kit and sold separately.Together, the control and components may be used as a comparison for success for new users or for quality control forFigure 2. Representative micrographs at (A,C) grid square level and (B,D) hole level of two different VitroEase Apoferritin Standard experienced users. batches prepared with the recommended vitrification settings.Cryo-EM sample preparation thermofisher.com/proteinbiology 115'