b'Table 2. Expression system selection guide.ExpressionHuman (HEK293) Hamster (CHO) Insect (Sf9, Sf21) Yeast Bacterial Cell-freesystemFeaturedMembrane proteins (GPCRs, ion channels,Ig-related proteins Intracellular proteins Industrial enzymes Bacterial proteins Rapid protein expressionapplication other membrane proteins)Other Difficult-to-express proteinsMembrane proteinsToxic proteins Low-complexity proteins Low-complexity proteinsToxic proteinsapplicationsSecreted proteinsSecreted proteinsMulti-protein complexesIn vitro labelingPosttranslationalFull Nearly full Partial Partial Limited PartialmodificationsProtein yield Up to 1 g/L Up to 3 g/L Up to 900 mg/L Up to 10 g/L Up to 10 mg/L Up to 750 mg/LRecommendedExpi293 expression system kits ExpiCHO Expression System Kit ExpiSf Expression SystemEasySelect Pichia Expression Kit Champion pET SUMO Expression System Cell-free in vitrohost system kit Starter Kit pBAD TOPO TA Expression KitExpi293F Cells ExpiCHO-S Cells (cGMP Banked) ExpiSf9 Cells PichiaPink Expression Strain Set One Shot BL21 Star (DE3) Chemically HeLa and CHO extractsRecommendedExpi293F GnTI Cells ExpiCHO-S Cells GS115, Pichia pastoris Yeast Strain Competent E. coli cellscells Expi293F Inducible Cells X-33, Pichia pastoris Yeast Strain BL21-AI One Shot Chemically Expi293F Inducible GnTI Cells Competent E. coli cellsRecommendedExpi293 Expression Medium ExpiCHO Expression Medium ExpiSf CD Medium PichiaPink Media Kit MagicMedia E. coli Expression Medium None requiredmedia YPD Broth LB BrothRecommendedExpiFectamine 293 Transfection Kit ExpiFectamine CHO Transfection Kit ExpiFectamine Sf TransfectionNeon Transfection System Starter Pack None required None requiredtransfectionReagentand deliveryRecommendedpcDNA3.4 TOPO vector pcDNA3.4 TOPO vector pFastBac 1 vector pPINK (LC and HC) Champion pET SUMO vector T7 cell-free expression vectorsexpressionpFastBac Dual Expression Vector pPICZ A, B, and C Pichia Vectors pBAD TOPO vectorvectors pPICZ A, B, and C Pichia Vectors pRSET bacterial expression vectorsNalgene shake flasks Nalgene shake flasks Nalgene shake flasks Nalgene shake flasks Nalgene shake flasksRecommendedNunc bioreactor tubes Nunc bioreactor tubes Nunc bioreactor tubesculturewareNunc plates Nunc plates Nunc platesRecommendedM-PER Mammalian ProteinM-PER Mammalian ProteinI-PER Insect Cell ProteinY-PER Yeast Protein Extraction Reagent B-PER Complete Bacterial Protein None requiredextractionExtraction Reagent Extraction Reagent Extraction Reagent Extraction Reagentreagent kitExpi293 Met () Protein Labeling KitRecommendedL-Methionine (Methyl-13C)protein labelingL-SelenomethionineExpi293 Expression Medium ExpiCHO Stable Production Medium ExpiSf CD Medium Bacto CD Supreme FermentationSingle-use bioreactor vessels EfficientFeed C+ 2X Supplement Single-use bioreactor vessels Production Medium (FPM)Scale-up Efficient-Pro Feed 2 Single-use bioreactor vesselsSingle-use bioreactor vesselsServices option Gene-to-protein services Gene-to-protein services Gene-to-protein servicesCloning and protein expression thermofisher.com/proteinbiology 9'