b'Table 7. Thermo Scientific Zeba resin selection guide by protein recovery and small molecule removal.7K MWCO 40K MWCOSize Recovery Removal Recovery RemovalPeptide/protein 7 kDa NR* NRProtein 713 kDa Protein 1420 kDa + +Protein 20150 kDa + +Molecule 500 Da + +Molecule 6001,200 Da +Molecule 1,2001,500 Da + Molecule 1,5002,000 Da NR +* NR: No recovery or removal.Table 8. Comparison of recommended sample volume capacities of common spin desalting products.0 mL 0.01 mL 0.1 mL 0.5 mL 1 mL 2 mL 3 mL 4 mLZeba micro 0.5 mL ZebaZeba spin desaltingspin column spin column 2 mL Zeba spin column 10 mL Zeba spin columnproducts5 mL Zeba spin columnPD SpinTrapG-25 columnCytiva productsPD MiniTrapPD MidiTrap PD-10G-25 column G-25 column Desalting columnsMicro Bio-Spin6 columnBio-Rad productsBio-Spin6 column1.5 mL 2.5 mL 3.5 mL Figure 6. Zeba Spin Desalting Columns result in high protein recovery while providing minimal sample dilution over a wider range 0.04 mg/mL BSA of sample concentrations and volumes compared to alternative products. Zeba Spin Desalting Columns, 10 mL (7K MWCO), and Cytiva PD-10 Columns were used to desalt 1.5, 2.5, and 3.5 mL BSA samples 0.2 mg/mL BSAat concentrations of 0.04, 0.2, and 1 mg/mL. Desalting was performed according to the manufacturers recommended protocols; both the spin 1.0 mg/mL BSA and gravity protocols were used for the Cytiva PD-10 Columns. Protein recovery was analyzed by SDS-PAGE. For each electrophoresis gel, an Load aliquot of starting sample equal to 1 g of BSA was loaded in lane 1 as Cytiva spin Cytiva gravity Cytiva spin Cytiva gravity Cytiva spin Cytiva gravityThermo Fisher Scientic Thermo Fisher Scientic Thermo Fisher Scienticthe loading control; all other desalted samples were loaded in the gel at the same volume as the loading control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting. The largest differences in recovery and concentration were noticed in the outlined area.For more information, or to view additional products, go to thermofisher.com/desaltingProtein sample preparation thermofisher.com/proteinbiology 21'