b'sSingle-particle analysis MicroED Cryo-ETProtein expression Cell growthMammalian cell expressionCell-free expressionProtein extraction and isolationCell lysisDetergents for protein solubilizationProtein purificationRecombinant protein purificationBiotin affinity purificationNanoDrop spectrophotometersProtein clean-upZeba spin desalting columnsProtein concentratorsCryo-EM standards and training Crystal growthVitroEase Apoferritin StandardVitroEase Training KitEM sample optimizationVitroEase Buffer Screening KitEM-compatible detergentsGrid selectionNegative stain screeningVitroEase Methylamine VanadateVitroEase Methylamine TungstateVitrification Vitrobot Mark IV SystemLamellae preparationCryo-FIBsCryo-EMTundra Cryo-TEM Krios G4 Cryo-TEMGlacios 2 Cryo-TEMFigure 1. Sample preparation workflows for high-resolution cryo-EM.114 Cryo-EM sample preparation thermofisher.com/proteinbiology'