b'Immunoprecipitation (IP), co-IP, and pull-down using magnetic beadsFast, reproducible sample processing with high yield and low nonspecific bindingMagnetic beads are the most rapidly growing method for IP and pull-down assays because they are faster, easier, and more efficient for pulling down proteins than nonmagnetic methods. We offer a wide variety of conjugated magnetic beads, including the highly referenced Invitrogen Dynabeads magnetic beads and the economical Thermo Scientific Pierce magnetic beads or magnetic agarose, to meet most application and budget needs.Highlights:Low backgroundlittle to no nonspecific binding, and no preclearingHighly sensitivemagnetic beads are the ideal choice for sensitive applications, such as IP of low-abundance proteinsAntibody savingsall binding occurs on the smooth outer surface of the beads, conserving precious antibodies and providing a solution that is cost-efficient per sampleFast and easymagnetic beads offer a rapid IP protocol, with no centrifugation or preclearing steps (Figure 2)Flexibleproducts for IP, co-IP, and pull-down assays; ideal for both manual and automated protocolsCompatiblemagnetic beads can be used in multiple workflows, including western blotting, mass spectrometry, and qPCR (for ChIP analysis)Automation readyDynabeads products for IP can be optimized with a Thermo Scientific KingFisher purification system to decrease hands-on preparation time and increase sample output (Figures 3 and 4)Start with:Figure 3. Thermo Scientific KingFisher Apex instrument.CD81KingFisher KingFisher Apex Flex ManualkDa5036 2616Std I II III I II III I II IIIFigure 4. IP of CD81 using manual methods vs. Thermo Scientific KingFisher Flex and Apex instruments. Jurkat cells expressing CD81 were lysed and incubated with Dynabeads Protein G coated with anti-CD81 antibody using manual and automated methods in triplicate. The samples were prepared for electrophoresis and western blotting, Figure 2. Immunoprecipitation in 40 minutes. Dynabeads magneticand labeling was performed using the anti-CD81 antibody.beads precoupled with protein A or protein G act as a suspendable solid support that can be fixed by the use of a magnet. This allows for simple and efficient antibody capture, followed by immunoprecipitation of pureFor more information about the KingFisher automation system, or target peptides, proteins, protein complexes, or other antigens. to schedule a demo, go to thermofisher.com/kingfisherapexProtein purification thermofisher.com/proteinbiology 35'