b'Protein concentratorsEasy-to-use devices for rapid and efficient concentrationThermo Scientific Pierce Protein Concentrators are easy-to-use centrifugal devices that provide fast processing and excellent recovery of protein samples. These disposable ultrafiltration devices are available in four volume capacities (0.5, 6, 20, and 100 mL) and contain a polyethersulfone (PES) membrane in five distinct molecular weight cutoffs (MWCOs): 3K, 5K, 10K, 30K, 50K, and 100K, for the concentration, desalting, and buffer exchange of biological samples, such as tissue culture media and chromatography fractions, or to remove unincorporated label following protein bioconjugation reactions.Highlights:Rapid processingunique design minimizes membrane foulingHigh recoveryretain 90% of protein samples while removing contaminants or exchanging buffers (Figure 7)Convenientclear markings, wide sample chamber, and removable filtrate chamber make handling simple and easy10090 99 98 99 98Protein recovery (%)8070 726050403020 271001 mg/mL 0.05 mg/mL 1 mg/mL 0.05 mg/mL 1 mg/mL 0.05 mg/mLThermo Scientic Millipore PallFigure 7. Pierce Protein Concentrators provide exceptional recovery of low-concentration samples. Samples of aprotinin (~6.5 kDa) were centrifuged in a Pierce Protein Concentrator, PES (3K MWCO, 0.5 mL), and other suppliers concentrators at 15,000 x g until the sample volume was 1/15 of the original volume. Protein recovery was determined using the Pierce BCA Protein Assay Kit.For more information, or to view additional products, go to thermofisher.com/concentrators22 Protein sample preparation thermofisher.com/proteinbiology'