b'Table 1. Overview of ion exchange, affinity, and activated supports.Application Purity level Ligand and/or chemistry Base bead type Packaging optionsIon exchangeMedium to highStrong anion exchange POROS Loose resinpurification (application-specific) Strong cation exchangeProtein A, protein G, protein A/G Agarose, magnetic beads, Antibodymagnetic agarose, POROS Loose resins or beads, spin columns and kits, purification High Protein L Agarose, magnetic beads chromatography cartridges, 96-well spin platesMelon Gel AgaroseNi-NTA, Ni-IDA, cobalt, glutathione Agarose, Superflow, magneticLoose resins or beads, spin columns and kits, Fusion protein High beads, magnetic agarose chromatography cartridges, 96-well spin platespurification Antic-Myc, anti-HA, anti-FLAG Agarose, UltraLink magnetic beads Loose resins or beads, kitsBiotin affinity High Avidin, streptavidin, NeutrAvidin,Agarose, magnetic beads Loose resins, spin columns and kits, purification monomeric avidin chromatography cartridges, 96-well spin platesAmine-reactive, sulfhydryl- Protein reactive, carbonyl-reactive,Agarose Loose resins or dry powderimmobilization High carboxyl-reactiveEpoxy, tosyl-activated, carboxylicMagnetic beads Loose beadsacid, amineTable 2. Select your resin based on purification scale and application.Scale High-throughput screening High-throughput batch Batch Pilot ProcessDescription Small scale, automationLab or bench scale Lab or bench scale Scale-up desired Production compatible scaleYield Microgram Milligram Milligram Gram KilogramMagnetic particle processor, Gravity flow, spin column (agarose),FPLC at mediumFPLC at high Format Magnetic particle processor 96-well spin plate (agarose) fast protein liquid chromatographyflow rates flow rates(FPLC) at low flow ratesHigh-throughput screening,High-throughput screening, interactionStructural analysis,Bulk Application interaction studies (IP, co-IP,studies (IP, co-IP, pull-down),Functional assays, structural analysis intermediate-scaleproductionpull-down), mutational analysis mutational analysis requiring mg scale productionMagnetic bead(12.8 m)Magnetic agarose(1040 m)Agarose(45165 m)RecommendedSuperflowresin type (45165 m)UltraLink resin(5080 m)POROS resin(50 m)Protein purification thermofisher.com/proteinbiology 31'