b'Instant ELISA: the one-wash ELISA kitReduce steps and hands-on time by as much as 60% Highlights:The one-wash Instant ELISA kit requires fewer handling stepsTime savingonly requires about 15 min for setupthan the conventional ELISA kit (Table 4). The Instant ELISA kitMaximum accuracyno need to add antibody or perform contains all of the necessary components, including captureserial dilution of standards; reduced handling means less error antibody and lyophilized detection antibody, streptavidin-HRP,and more consistent resultsand sample diluent. Additionally, the strip wells containing theBetter valuegenerate standard curve data in parallel with standards for the standard curve are provided separately; theyadditional well strips provided, to enable use of all 96 wells for are ready to use, helping save both time and wells. Less hands- your sampleson time can help increase productivity, which leaves more time for your research. Switching to Instant ELISA kitsThe Instant ELISA kits utilize the same antibody pairs for many Table 4. Steps for conventional ELISA vs. Instantanalytes across all assay formats. This enables cross-platform ELISA kits.performance and comparable data using any of our Instant ELISA 17 steps for7 steps for kits, ELISA, and ProcartaPlex multiplex assays. For example, if conventional ELISA Instant ELISA kits you are currently using an Invitrogen sCD44var Coated ELISA, you can be confident in switching to the Invitrogen sCD44var 1 Washing of coated plate Instant ELISA assay, knowing that the data generated across the 2 Reconstitution of standardtwo kits will remain consistent (Figure 2).protein3 Addition of sample diluent to standard wells4 Titration of standard curve 1 Rehydration of platesCD44var Coated ELISA (ng/mL)5 Addition of sample diluent 2 Sample addition 80 R 2= 0.98706 Sample addition 3 Incubation 60507 Dilution of biotin conjugate 40308 Addition of biotin conjugate 20109 Incubation 00 10 20 30 40 50 60 7010 Preparation of streptavidin- sCD44var Instant ELISA kits (ng/mL)HRP conjugate Figure 2. Performance comparison of human sCD44var Instant 11 Washing step ELISA to coated ELISA. Human sCD44var Instant ELISA kit was tested against a human sCD44var Coated ELISA kit. A coefficient of 12 Addition of streptavidin- determination (R) of 0.98 was observed using 16 serum samples, HRP conjugate demonstrating comparable performance of the Instant ELISA and coated ELISA kits.13 Incubation14 Washing step 4 Washing step15 Addition of TMB substrate 5 Addition of TMB substrate16 Addition of stop solution 6 Addition of stop solution17 Calculation of results 7 Calculation of resultsLearn more about Instant ELISA kits at thermofisher.com/instantelisaImmunoassays thermofisher.com/proteinbiology 77'