b'Primary antibodiesQuality antibodies validated* and cited in many applicationsThe Invitrogen antibody portfolio has more than 200,000 antibodies cited across various applications including flow cytometry, IHC, ICC/IF, western blotting, ELISA, IP, and other applications, supporting over 50 research areas. Our primary antibody portfolio offers a broad range of choices with high-quality recombinant monoclonal, monoclonal, and polyclonal antibodies that cover greater than 91% of the proteome. With over 375,000 publications and 835,000 data images and counting, we have the antibodies you can trust.Highlights:Quality antibodiescontinued advanced specificity testing to ensure they bind to the right target (Figure 6)Purchase with confidenceuse our enhanced search experience to filter and compare, so you find the data and information you needMore choicesour broad portfolio allows you to easily find the antibodies you need to support your researchCommitment to antibody specificity and reproducibilityThermo Fisher Scientific is working to redefine antibody performance standards with a comprehensive approach to how antibodies are evaluated and validated. Specificity testing is combined with extensive application validation data to provide confidence that our high-quality Invitrogen antibodies will help enable superior performance in your research.You can easily identify antibodies that have already undergone this Advanced additional testing with the Advanced Verification badge. This badge can Verification be found in antibody search results and on appropriate antibody product web pages. The additional data supporting the Advanced Verification status can be found in the product-specific data image galleries.A-431 control A-431 Cas9 A-431 EGFR KO Figure 6. Western blot analysis using primary antibodies and+ fluorescent dyeconjugated secondary antibodies. Western blot analysis of phospho-EGFR and phospho-MEK was performed by loading ~160 kDa p-EGFR 30 g of the following whole-cell extracts: A-431 control (lane 1), A-431 treated with EGF (lane 2), A-431 Cas9 (lane 3), A-431 Cas9 treated with EGF (lane 4), A-431 EGFR KO (lane 5), A-431 EGFR KO treated with ~160 kDa EGFR EGF (lane 6). Phospho-EGFR was detected at ~160 kDa using rabbit polyclonal antibody, EGFR was detected at ~160 kDa using mouse ~45 kDa p-MEK1 monoclonal antibody, phospho-MEK1 was detected at ~45 kDa using rabbit monoclonal antibody, and GAPDH was detected at ~37 kDa using goat polyclonal antibody. Target proteins were detected using Invitrogen ~37 kDa GAPDH Donkey AntiMouse IgG Alexa Fluor Plus 680 Conjugate, Invitrogen Donkey AntiRabbit IgG Alexa Fluor Plus 800 Conjugate, and Invitrogen Donkey AntiGoat IgG Alexa Fluor Plus 555 Conjugate.* The use or any variation of the word validation refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic use.Search for antibodies or find out more at thermofisher.com/antibodiesWestern blotting thermofisher.com/proteinbiology 67'