b'VitroEase Buffer Screening KitThe Thermo Scientific VitroEase Buffer Screening Kit provides preformulated buffers and detergents with a comprehensive screening strategy to identify optimal protein conditions for cryo-EM SPA.Highlights:Ease of usepreformulated buffers and detergents at 10X concentration Comprehensive14 buffers and 6 detergents commonly used for cryo-EM sample preparation provided with an optimized screening strategy Flexiblerange of salts, pH, and detergent formulations for sample optimization of 400 gridsValidatedfunctionally validated using cryo-EM SPACryo-EM sample screening evaluates protein behavior in various buffers and additive conditions with the end goal of identifying the optimal conditions in which the protein is stable, randomlyPuried protein Sample prepMicroscopyoriented, and equally distributed in ice. Kit buffers are provided with a pH range from 3.6 to 8.9, premixed with various salts andSelecting bu\x0eers and detergentsBiochemistry optimizationdivalent cations.The comprehensive sample screening strategy uses twoAdding bu\x0eers/detergents to the proteinrounds of screening and 24 grids, taking approximately twoVitribot EPU Multigriddays to process (Figure 3). In the first round, optimal proteinCryo-EM grid vitricationconcentration, buffer, and pH conditions are determined. In the second round, detergents are further optimized. A condensedFirst screening roundstrategy is also provided using 8 grids and taking one day to process, which can be used when little sample is available or forSecond screening rounduse with side-entry or semiautomated sample loading systems like the Thermo Scientific Tundra Cryo-TEM.Figure 3. Comprehensive cryo-EM sample screening strategy.The recommended screening strategies are designed for use with the Thermo Scientific Vitrobot Mark IV System and Thermo Scientific Krios, Glacios, and Talos Arctica microscopes with autoloader systems and EPU multigrid.116 Cryo-EM sample preparation thermofisher.com/proteinbiology'