b'Tandem Mass Tag (TMT and TMTpro) systemsSimultaneously identify and quantify protein expression from multiple samples in a single analysisThe Thermo Scientific TMT and TMTpro Label Reagent Sets enable simultaneous quantitation of up to 11 (TMT reagents) or 18 (TMTpro reagents) samples, providing the highest throughput available for protein identification and quantitative analysis by tandem mass spectrometry (MS).Highlights:Multiplexconcurrent MS analysis of up to 1118 samples derived from cells, tissues, or biological fluids (Figures 13)Robustincreased multiplex capability results in fewer missing A. TMT Reagent Generic Chemical Structurequantitative values among samples and higher confidence among replicates HCD OO OEfficientamine-reactive, NHS esteractivated reagentsN N O Nensure efficient labeling of all peptides regardless of protei ETD HnOsequence or proteolytic enzyme specificityMass Mass Amine-ReactiveReporter Normalizer GroupConvenientprovided in a ready-to-use, single-use format to ensure optimal stability and performance or in bulk for custom formatting 10 6A. TMT10plex Reagents (TMT ) B. TMTsixplex Reagents (TMT)A O O O B O O O * O O O126 N * * * O N N * * * * * O N * N * N * O N* N * NH 126 H 129 HO O * OO O O O O O O O O * * O O O127N * N N O N N * * * * * O N 127C * N * N * * * O N * N * O N* * * * N NH H 127 H 130 HO O O * OO O O * O O O * O O O * * O O O128N * N * N * * * O N N * * * O N 128C N * * * O N * * N N O NN NH O H O 128 H O 131 H O* * ** O * OO O O O129N * N N N * N 129C* N * O * * N OH H* O * O* O O O * * O O O130N * N * * N O N * N * O N 130CN* H O * H O* * O O O131 * * N N O N* H OFigure 1. Chemical structures of TMT reagents. (A) Structures of TMT10plex reagents with C and N heavy-isotope positions (red asterisks). (B) Structures of TMTsixplex reagents with C and N heavy-isotope positions (red asterisks).90 Mass spectrometry thermofisher.com/proteinbiology'