b'Rapid transfer systemsPower Blotter systemsThe Invitrogen Power Blotter is our flexible solution for western blot transfer. Designed for rapid 510 minute semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes, the Power Blotter contains an integrated power supply, LCD touchscreen, and preprogrammed, optimized transfer protocols. Harness the power of the Power Blotter in your western blotting workflow.Highlights:User-friendlyall-in-one transfer system, with an integrated power supply and touchscreenVersatileour most flexible transfer device, with options in throughput, consumables, and transfer speed, designed to grow around your labs needs Efficiencyhigh transfer efficiency for a broad range of protein sizes (Figure 1)Power Blotter with Pre-cut Membrane and FiltersPower Blotter with Select Transfer Stack and Power Blotter 1-Step Transfer BufferKLH KLHEGFR EGFRHsp90 Hsp90PDI PDIEGFR EGFR(extra band) (extra band)Cyclophilin B Cyclophilin BFigure 1. Power Blotter Select Transfer Stacks and Power Blotter Pre-cut Membrane and Filter stacks efficiently transfer high, medium, and low molecular weight proteins. Western blot analysis of KLH, EGFR, Hsp90, PDI, and cyclophilin B was performed by loading serial dilutions of KLH-spiked HeLa lysate with KLH spike (starting at 7.5 g HeLa lysate, 7.5 g KLH spike per well, serially diluted 2:3) into Invitrogen Bolt 412% Bis-Tris Plus gels. Proteins were transferred for 7 minutes using a Power Blotter Select Transfer Stack (left) or Power Blotter Pre-cut Membrane and Filter stack (right), and then probed with target-specific primary antibodies and fluorescently conjugated secondary antibodies. Images were captured using automatic exposure on an Invitrogen iBright FL1000 Imaging System.View the how-to video:Learn more at thermofisher.com/powerblotter62 Western blotting thermofisher.com/proteinbiology'