b'Slide-A-Lyzer dialysis productsEasy-to-handle devices, cassettes, and flasks for secure sample processingThermo Scientific dialysis units facilitate rapid and trouble-free dialysis of sample volumes from 10 L to 250 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss. Thermo Scientific Pierce 96-Well Microdialysis Plates and Slide-A-Lyzer MINI Dialysis Devices are ideal for small volumes, Slide-A-Lyzer G3 Dialysis Cassettes are recommended for small to medium volumes, and Slide-A-Lyzer Dialysis Flasks are recommended for larger volumes (Table 5).Highlights:Excellent sample recoverylow-binding plastics and membranes help minimize sample loss compared to filtration and resin systems, while maximizing dialysis speed (Figures 4 and 5)Convenienteasy-to-grip design helps simplify sample addition and removal with syringe or pipetteSecuresealed membranes help prevent leakage that can occur with dialysis tubing and homemade devicesValidatedeach device is leak-tested during productionTable 5. High-performance dialysis product selection guide.101,000 L 102,000 L 1125 mL 150250 mL 15100 mLThermo Scientific MembranePierce MicrodialysisSlide-A-Lyzer MINISlide-A-Lyzer G3Slide-A-Lyzer SnakeSkinMWCO PlateDialysis Device Dialysis Cassette Dialysis Flask Dialysis Tubing2K NA3.5K 7K NANA NA 10K 20K NA2K MWCO 3.5K MWCO 7K MWCO 10K MWCO 20K MWCO100 100 100 100 100 Vitamin B 12(1.4K MW)90 90 90 90 90 Bacitracin (1.4K MW)80 80 80 80 80 Tyrosine protein kinase (1.7K MW)70 70 70 70 70 Biotin-TPK (1.9K MW)% Retention % Retention % Retention % Retention % Retention60 60 60 60 60 Protein kinase Ce peptide (2K MW)50 50 50 50 50 Insulin A chain (2.5K MW)40 40 40 40 40 Insulin B chain (3.5K MW)30 30 30 30 30 Insulin (5K MW)20 20 20 20 20 Cytochrome c (12.4K MW)10 10 10 10 10 Lysozyme (14K MW)0 0 0 0 0 Myoglobin (17K MW)Figure 4. Sample retention by the Thermo Scientific Slide-A-Lyzer cassette membranes with 2K, 3.5K, 7K, 10K, or 20K molecular weight cutoff (MWCO). Individual proteins or vitamin B 12(1 mg/mL) in either saline or 0.2 M carbonate-bicarbonate buffer, pH 9.4, were dialyzed overnight (17 hr) at 4C. The amount of retentate was estimated using either the Pierce BCA Protein Assay Kit or absorption at 360 nm (for vitamin B 12 ).18 Protein sample preparation thermofisher.com/proteinbiology'