b'BiotinylationThe highly specific interaction of avidin with biotin is a valuable tool in designing nonradioactive purification and detection systems. The extensive line of Thermo Scientific biotinylation labeling reagents are designed to be conjugated to an antibody, protein, peptide, cell surface proteins, or nucleic acids (Table 4). Multiple types of biotinylation reagents are available with varied uses preferable for specific applications, so weve developed easy-to-use guides for selecting the right reagent. In addition to bulk packaging, we offer the easy-to-use No-Weigh format and optimized kits of our biotinylation reagents.Highlights:Multiple reactivitieswide selection of amine-, sulfhydryl-, carbonyl-, UV-, and click-reactive biotinylation reagentsReversible and cleavabledesthiobiotin and cleavable biotin for capture and release applications Application-specificreagents designed for specific applications such as cell surface protein labeling or site-specific antibody labelingTable 4. Recommended biotinylation reagents for specific targets and applications. Target Example Reactivity ApplicationSulfo-NHS-LC-biotin Amine-reactiveAntibody Biotin-PEG -hydrazide Carbohydrate-reactive ELISA, blotting, imaging4SiteClick Biotin Antibody Labeling Kit Azide- and alkyne-reactiveSulfo-NHS-SS-biotin Amine-reactivePurified protein Maleimide-PEG-biotin Sulfhydryl-reactive ELISA, blotting, or affinity purification of receptor or antigenBiotin-PEG -hydrazide Carbohydrate-reactive4NHS-PEG -biotinAmine-reactive4 ELISA, blotting, or affinity Purified peptide Maleimide-PEG 2 -biotin Sulfhydryl-reactive purification of receptor or antigenAmine-PEG -biotin Carbohydrate- and amine-reactive2Cell surface proteins Cell Surface Protein Isolation Kit Amine-reactive Affinity purification or removal of cell surface receptor ligandsDNA/RNA orPsoralen-PEG-biotinRandom Capture or detection of oligonucleotides Amine-PEG-biotin Carbohydrate- and amine-reactive oligonucleotides in ELISA-type applications or affinity purificationLearn more at thermofisher.com/biotinlabeling106 Protein bioconjugation, crosslinking, and modification thermofisher.com/proteinbiology'