b'Novex Tris-glycine gelsLoad up to 60 L of sampleThe Invitrogen Novex Tris-Glycine mini gels, WedgeWell format, and Invitrogen Novex Tris-Glycine Plus midi gels are polyacrylamide gels based on traditional Laemmli chemistry that enable the use of Laemmli sample and running buffers. Novex Tris-Glycine gels provide high-quality performance and separation of a wide range of proteins into well-resolved bands.Highlights:Wedge-shaped wellseasily load up to 60 L of sample without sacrificing gel width or length (mini gel only)High performanceexcellent protein band resolution and sharpness (Figure 3)Fast run conditionsquickly separate your proteins using constant voltage in less than 60 minutesFlexibleNovex Tris-glycine gels do not contain SDS and can be used to run proteins in native or in denatured form1 2 3 4 5 6 7 101 2 3 4 5 6 7 8 9 10 8 9 10 1 2 3 14 25 36 47 58 69 7 8 9 10Novex 420% TNovex 4ris-glycine mini gel,20% Tris-glycine mini gel, Bio-Rad TGX 4Bio-Rad TGX 420% gel20% gelWedgeWell formatWedgeWell formatFigure 3. Better protein resolution and band sharpness with Novex Tris-glycine mini gels, WedgeWell format. Protein ladder, purified proteins, and E. coli lysate were loaded on a gradient Novex 420% Tris-Glycine mini gel, WedgeWell format, and a Bio-Rad TGX 420% gradient gel. The Bio-Rad TGX gel displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, and 8 that are not seen in the Novex Tris-glycine gel. The Novex gel also displays better protein band sharpness and resolution of lysate than the Bio-Rad gel. Lanes 1, 10: 5 L Invitrogen Mark12 Unstained Standard; lane 2: 10 g E. coli lysate (10 L); lane 3: 6 g catalase (10 L); lane 4: 6 g carbonic anhydrase (10 L); lane 5: 6 g lysozyme (10 L); lane 6: 6 g hIgM (10 L); lane 7: 6 g BSA (10 L); lane 8: 6 g -galactosidase (10 L); lane 9: 20 g E. coli lysate (20 L).Find out more at thermofisher.com/novexwedgeProtein gel electrophoresis thermofisher.com/proteinbiology 47'