b'A 100 B 1.25 C 100Dialysis device in conical tube 90 2KConventional dialysis 1.00 80 3.5KResidual NaCl (%)Residual NaCl (M) Residual NaCl (%)75 70 10K0.75 60 20K50 500.50 4030250.25 20100 0 00 1 2 3 4 5 0 1 2 3 4 5 0 2 5 10 15 20 25Time (hr) Time (hr) Time (hr)Figure 5. The rate of removal of NaCl using various dialysis products. NaCl removal from samples was determined by measuring the conductivity of the retentate at the indicated times. (A) Slide-A-Lyzer MINI Dialysis Device (10K MWCO, 2 mL) vs. conventional dialysis. Bovine serum albumin (BSA) samples (2 mL, 0.25 mg/mL in 1 M NaCl) were dialyzed against 45 mL of water in 50 mL disposable conical tubes on an orbital shaker (300 rpm) at room temperature. The water was changed once after 2 hr. Results are the average of two samples. For conventional dialysis, the samples were dialyzed against 2 L of water in a beaker with stirring. More than 95% of NaCl was removed within 4 hr. (B) Samples of 0.1 mL of 0.4 mg/mL cytochrome c in 1 M NaCl were dialyzed in the Pierce 96-Well Microdialysis Plate against 1.8 mL of water at room temperature with gentle shaking. The buffer was changed hourly over a 4 hr period. Removal of NaCl was 83% after 2 hr and 99% after 4 hr. (C) Proteins in 200 mL samples containing 1 M NaCl were dialyzed at room temperature using Slide-A-Lyzer Dialysis Flasks with 2K, 3.5K, 10K, or 20K MWCOs. The dialysis buffer (4 L) was changed after 2 and 5 hr (triangles) and at 41 hr for the flask with 2K MWCO. More than 95% of NaCl was removed within 8 to 18 hr (41 hr for the flask with 2K MWCO).How-to videoWatch the video to see how to use Slide-A-Lyzer G3 dialysis cassettes in a typical dialysis protocol.For more information, or to view additional products, go to thermofisher.com/dialysisProtein sample preparation thermofisher.com/proteinbiology 19'