b'ProQuantum high-sensitivity immunoassay kitsThe ProQuantum high-sensitivity immunoassay is a platform innovation thatHighlights:provides researchers with an easy-to-run, high-performance assay with noHigh-sensitivitydetect low levels of proteins proprietary instrument to purchase. By utilizing proximity-based amplificationwith greater sensitivity than with traditional technology, we have combined the analyte specificity of high-affinityELISA methodsantibodyantigen binding with the signal detection capabilities of real-timeBroad dynamic range5 orders of magnitude, PCR, to achieve a highly sensitive protein quantitation assay with a largeminimizing sample dilutions to help ensure they fall dynamic range. With its ability to detect even lower levels of protein thanwithin the range (Figure 5)traditional methods with very small sample consumption, you can get theSmall sample consumptionuse 25 L of most out of your precious or limited samples. sample (for example, 2 L vs. 75 L for triplicate wells with other methods)Heres how it works Fast, easy workflow2 hr from sample to answer, The ProQuantum immunoassays utilize a matched pair of target-specificno wash steps, and a single 1 hr incubationantibodies each conjugated to a DNA oligonucleotide. During antibodyanalyte binding, the two oligos are brought into close proximity,No proprietary instrument to purchaseruns on any real-time PCR instrumentwhich then allows for ligation of the two strands and subsequent creation of a template strand for amplification. This proximity-based technologyIncludes intuitive analysis softwareleverages the sensitivity and large dynamic range of Applied Biosystemscomprehensive data analysis and statistical TaqMan real-time PCR technology (Figure 4). groupwise comparison35PL regression3Fluorescence t3 oligo 5 oligo 5 5CTargetTarget Target Cycle number Protein conc.A BA BFigure 4. How ProQuantum immunoassay works. (A) Antibody conjugates binding to target (1 hr incubation). (B) Ligation and amplification of signal (in qPCR instrument).16 4 16 414 2 14 212 12 1t 10 1 C t t 10C C 8 450 A C 8 0.5 450 A6 0.5 6 0.254 0.25 42 2 0.1250 0.125 0 0.06250.01 0.1 1 10 100 1,000 10,000 0.01 0.1 1 10 100 1,000 10,000IFN, pg/mL IL-8, pg/mL16 4 16 414 2 1412 12 210 1 10 1t 8 0.5 450 A 8 450 A6 6 0.50.254 4 0.252 0.125 20 0.0625 0 0.1250.01 0.1 1 10 100 1,000 10,000 0.01 0.1 1 10 100 1,000 10,000IL-1, pg/mL IL-6, pg/mLFigure 5. Superior sensitivity and broader dynamic range. Standard curves for Invitrogen ProQuantum High-Sensitivity Human IFN, IL-8, IL-1, and IL-6 immunoassay kits (red) show larger dynamic range and improved separation at the low end of the curve for greater sensitivity than normal and ultrasensitive ELISAs (purple).Find out more and select your assay at thermofisher.com/proquantum80 Immunoassays thermofisher.com/proteinbiology'