b'Mass Mass Amine-reactivereporter normalizer groupCleavablelinkerTMTpro-126 TMTpro-127NTMTpro-127C TMTpro-128NTMTpro-128C TMTpro-129NTMTpro-129C TMTpro-130NTMTpro-130C TMTpro-131NTMTpro-131C TMTpro-132NTMTpro-132C TMTpro-133NTMTpro-133C TMTpro-134NFigure 2. Structures of TMTpro 18plex reagents with 3C and 5N heavy-isotope positions (red asterisks).Label Combine Fractionate, Data analysis and Sample 1 TMTpro 126 clean up, quantitation Treat Denature, perform using Proteome Sample 2 samples reduce, LC-MS/MS Discoverer softwareTMTpro 127N analysisand isolate alkylate, do: proteins tryptic digest :: :: :Sample 16 TMTpro 134NFigure 3. Procedure summary for MS experiments using TMTpro isobaric mass tagging reagents. Protein extracts isolated from cells or tissues are reduced, alkylated, and then digested using the Thermo Scientific EasyPep Mini MS Sample Prep Kit or an equivalent method. Samples are then labeled with the TMTpro reagents before sample mixing, fractionation, and cleanup. Labeled samples are analyzed on a high-resolution Thermo Scientific Orbitrap LC-MS/MS mass spectrometer before data analysis to identify peptides and quantify relative abundance of reporter ions.Learn more at thermofisher.com/tmtproMass spectrometry thermofisher.com/proteinbiology 91'