b'Overview of western blot transfer systemsWhen choosing a transfer methodology, convenience, speed,come in a variety of capacities and designs and typically offer flexibility, reproducibility, and throughput are importantthe greatest flexibility in throughput, but this attribute should considerations (Table 1). While the devices the laboratorybe weighed against the transfer speed and potential handling currently has may be the most convenient, consider howinconsistencies. With respect to transfer efficiency, these modern alternative methods may improve steps such as transfertransfer systems will transfer proteins of typical molecular weights preparation and cleanup. A rapid dry transfer system, whichwith high efficiency. Our semi-dry systems and dry system offer utilizes a unique gel matrix that incorporates buffer ions insteadpreprogrammed optimized methods for particular molecular of transfer buffersoaked filter papers, with ready-to-useweight ranges, including high and low molecular weights. consumables, provides greater convenience, requiring very littleTransferring very high or very low molecular weight proteins often setup and cleanup time. Regarding transfer speed, wet transferrequires optimization regardless of the system used. However, systems are typically the slowest, followed by conventionalbecause of the flexibility of wet transfer (e.g., transfer membranes semi-dry transfer (using Towbin buffer), with dry transfer systemsof different pore sizes can be swapped easily and transfer buffer being the fastest. However, with the specially designed transferformulations can be modified), wet tank transfer is a good place buffers for semi-dry systems, semi-dry systems can matchto start when transferring proteins of very high or low molecular the speeds offered by dry transfer systems. Wet tank systemsweights.Table 1. Characteristics of Invitrogen transfer systems.Wet transfer Semi-dry transfer Dry transferMini BlotSureLock TandemPower BlotteriBlot 2 Gel Transfer Module Midi Blot Module Systems DeviceCapacity 1 mini gel per blot1 midi gel per blot14 mini or12 mini or module; 12 blotmodule; 12 blot12 midi gels 1 midi gelmodules per tank modules per tankTransfer time 60 min 30 min 710 min 7 minBlotting area 9 x 9 cm 9.2 x 14.4 cm 10 x 18 cm or 8.5 x 13.5 cm21 x 22.5 cmTransfer buffer volume 220 mL per blot module 300 mL per blot module Pre-cut membranesBuffer not requiredand filters: 50100 mL; pre-assembled Select transfer stacks: buffer not requiredPower supply External External Internal InternalRequired equipment Mini Gel Tank SureLock Tandem Midi Gel TankLearn more about the western blot transfer process in the Western Blotting Handbook at thermofisher.com/westernhandbookWestern blotting thermofisher.com/proteinbiology 59'