b'Table 3. Selection guide for protein ladders and standards.No. ofCategory Product Advantages Range bands Recommended forUnstained ladders and standardPageRulerSharp bands and precise molecular Unstained Lowweight estimation for low molecular3.4100 kDa 8 MW determination, gel staining, Range Proteinweight proteins chemiluminescent band visualizationLadderUnstainedPageRulerSharp bands and precise molecular standards Unstained weight estimation for a wide range of5250 kDa 14 MW determination, gel staining, Broad Rangeproteins chemiluminescent band visualizationProtein LadderHiMark UnstainedConvenient molecular weight MW determination (native gels), gel staining, Protein Standard estimation for high molecular weight201,200 kDa 8 chemiluminescent band visualizationproteinsPrestained protein ladders and standardFluorescence visualizationdetect the ten stained proteins using the 670 nm red laser or 700 nm channelWestern blotting: detection of the two of a fluorescence imager unstained bands using the detection iBright PrestainedWestern blot confirmationdetect11250 kDa 12 method for the target protein; compatible Protein Ladder the two unstained proteins using thewith chemiluminescent substrates and detection system for the target protein fluorescent secondary antibodiesReference band55 kDa band has greater intensity for easy orientationPageRulerOutstanding clarity for easy MW determination, protein band Prestained molecular weight determination of low10180 kDa 10 visualization, monitoring electrophoresis PrestainedProtein Laddermolecular weight proteins run and transfer, chemiluminescent standards band visualizationPageRuler PlusOutstanding clarity for easy molecularMW determination, protein band Prestained Proteinweight determination of a broad range10250 kDa 9 visualization, monitoring electrophoresis run Ladder of proteins and protein transferHiMark Prestained Superb analysis of high molecularMW determination (high MW proteins), Protein Standard weight proteins 30460 kDa 9 protein band visualization, monitoring electrophoresis run and protein transferSpectra MulticolorSuperior visualization and analysis of aMW determination, protein band visualization Broad Rangebroad range of proteins 10260 kDa 10 (best), monitoring electrophoresis run and Protein Ladder protein transferSpectra MulticolorSuperior and convenient visualization ofMW determination, protein band visualization High Range Proteinhigh molecular weight proteins 40300 kDa 8 (best), monitoring electrophoresis run and Ladder protein transferOther ladders and standardsChemiluminescent MagicMark XPAccurate molecular weight estimationMW determination, gel staining, standard Western Proteindirectly on western blots 20220 kDa 9 chemiluminescent band visualization (best)StandardNear-infrared (NIR) PageRulerSharp prestained standard for standard Prestained NIRnear-IR fluorescent visualization and11250 kDa 10 MW determinationProtein Ladder protein sizingFluorescent BenchMarkEfficient estimation of molecular weightMW determination, chemiluminescent standard Fluorescent Proteinby fluorescent detection 11155 kDa 7 band visualization StandardBenchMarkConvenient detection and protein sizingMW determination, gel staining, His-tag standard His-tagged Proteinof His-tagged proteins 10160 kDa 10 chemiluminescent band visualization (using Standard anti-6xHis antibody)IEF IEF Marker 310 Accurate determination of proteinpI 3.510.7 13 pI determinationisoelectric pointsProtein gel electrophoresis thermofisher.com/proteinbiology 53'