b'Secondary antibodiesBroad menu for multiple detection optionsOur selection of high-quality secondary antibodies can be used for detection of the primary antibody in a broad range of applications, including western blotting, ICC/IF, flow cytometry, and ELISA. These highly cited, well-characterized secondary antibodies are linked to dyes, enzymes, and labels: including enzyme conjugates, such as HRP and AP, labels such as biotin, and classic fluorescent dyes such as FITC, RPE, and APC. We are also the premier supplier for Invitrogen Alexa Fluor and Invitrogen Alexa Fluor Plus dyes.Fluorescent detectionwell suited for studies that need to address levels of protein expression or require simultaneous detection of more than one antigenChemiluminescent detectionwell suited for low-abundance proteins, and sensitivity can be further improved using our poly-HRPconjugated secondary antibodies or enhanced chemiluminescent (ECL) substrate to deliver high femtogramlevel protein detectionColorimetric detectionprovides quick and easy results and does not require special equipment 15.0 g 5.0 g 1.6 g 0.6 g 0.2 g 0.06 gWe offer a wide range of antibody conjugates targeting an array of hostsA 130 kDaand species derived from a number of sources, including a rich lineup of95 kDa Hsp90recombinant secondary antibodies that offer improved sensitivity. Additionally,70 kDa HDAC1Thermo Fisher is the only reagent provider offering Invitrogen Alexa Fluor55 kDaPlus conjugates (Figure 7). Our extensive menu includes 7 colors and43 kDa11 host/target species combinations.Highlights:Avoid cross-reactivityuse our secondary antibodies that are highlyB 130 kDacross-adsorbed against serum proteins or immunoglobulins of other species 95 kDa Hsp90Invitrogen Alexa Fluor and Invitrogen Alexa Fluor Plus secondary70 kDa HDAC1antibodiesget distinct fluorescent bands in multiplex experiments by55 kDaselecting nonoverlapping spectra for a variety of conjugates using our43 kDainteractive Fluorescence SpectraViewer toolFigure 7. Comparison of detection sensitivity. Sensitive detectionuse Alexa Fluor Plus secondary antibodies or the(A) Invitrogen Alexa Fluor Plus 800 Secondary catalytic activity of our horseradish peroxidase (HRP)-conjugated antibodiesAntibody produces more bands at higher dilutions, in conjunction with SuperBoost technology for superior detection ofdemonstrating greater sensitivity and range of low-abundance proteins detection compared to (B) another suppliers fluorophore-conjugated secondary antibody.Find out more at thermofisher.com/alexafluorplus68 Western blotting thermofisher.com/proteinbiology'