b'Luminex instrumentationThe Luminex family of instruments includes options for various multiplexing capabilities, throughput, and read times (Table 7). All instruments are capable of protein and nucleic acid applications, and all are supported by commercially available assays that can multiplex up to 80 biomarkers simultaneously. Table 7. Comparison of Luminex systems for multiplexing.Luminex xMAP INTELLIFLEXLuminex xMAPLuminex FLEXMAP 3DLuminex 200Instrument DR-SE System INTELLIFLEX System Instrument System Instrument SystemMost advanced Luminex platform Higher-throughputMost established platformmultiplexingApplications Protein and nucleic acid analysisMultiplex capacity Up to 500 targets Up to 100 targets(80 on MagPlex microspheres)Max panel size 80 biomarkersSensitivity0.061 pg/mL (immunoassays; assay and target dependent); 1,000 RNA copies per target/well (RNA assays)Dynamic range 5.5 orders of magnitude (RP1) 5.5 orders of magnitude4.5 orders of magnitude3.5 orders of magnitude 4.5 orders of magnitude (RP2) (RP1) (RP1) (RP1)Sample volume6.380 L 2580 L(assay dependent)Plate format 96-well 96-well384-wellRead time 20 min (96-well) 45 min (96-well)75 min (384-well)Footprint58.4 x 61 x 76 cm 110 x 62 x 63 cm 115 x 60 x 50 cmOptics/hardware Flow cytometrybased lasers, APDs, PMTsReporter laser/optics 532 nm (green) and532 nm (green) 532 nm (green) 532 nm (green)405 nm (violet)Operational validation/Calibration, verification, and fluidics compliance 21 CFR compliance (coming in 2022 for INTELLIFLEX instrument)Automation Automation integration software available (coming in 2022 for INTELLIFLEX instrument)Data analysis apps for Luminex assaysAnalytics (software) on the Thermo Fisher Connect Platform xPONENTbasic plus partner analyticsAccessories to keep your Luminex instruments running strong include sheath fluid, calibrator and control microspheres, verification and calibration kits, and hand-held plate washers.Learn more at thermofisher.com/luminexinstrumentsTest your protein research knowledge Question: Chromogenic ELISA assays (select all that are true): A. Are the most commonly used ELISA detection methodB. Enable kinetic studiesC. Are as sensitive as chemiluminescent ELISAsD. Use a standard absorbance plate readerAnswer: A, B, and D84 Immunoassays thermofisher.com/proteinbiology'