b'Chemiluminescent detectionOptimized substrates to enhance sensitivity and reduce backgroundThermo Scientific chemiluminescent HRP substrates offer excellent performance in western blotting applications with longer light emission and stronger signal intensity than other luminol-based detection systems. Choose the appropriate substrate for your needs from the Thermo Scientific Pierce ECL and Thermo Scientific SuperSignal families of chemiluminescent HRP substrates. As with other components in a western blotting system, there are many chemiluminescent substrate choices available. The appropriate substrate selection depends on the detection level (sensitivity) required, target protein abundance, and sample availability (Table 2).Highlights:Excellent sensitivitysubstrates providing picogram to attogram sensitivityStrong light emissionlonger signal duration allows for multiple exposuresHigh intensitysignal is twice as intense as other luminescence-based systemsAntibody savingsour substrates are optimized to work with more dilute primary and secondary antibodiesTable 2. Recommended chemiluminescent substrates for western blot detection using HRP.SuperSignal West Dura SuperSignal West Pico PLUS SuperSignal West AttoChoose when Performing quantitative westernPerforming everyday applications;Target is very low-abundance, blotting or maximum signaloffers improved sensitivity oversample is limited, or antibodies duration is needed base-level ECL are limitedFeatures Longest duration and linearityWidest dynamic range Highest sensitivityDetection level Mid-femtogram Low-picogram to femtogram Low-femtogram to high-attogramSignal duration 24 hours Up to 24 hours 6 hoursRecommendedPrimary: 1:5,000 Primary: 1:1,000 Primary: 1:5,000antibody dilutionsSecondary: 1:50,0001:250,000 Secondary: 1:20,0001:100,000 Secondary: 1:100,0001:250,000(1 mg/mL stock)Western blotting thermofisher.com/proteinbiology 69'