b'Protein crosslinkingOur crosslinking reagents are designed for a variety of techniques10including proteinprotein conjugation, protein immobilization, Degree of labeling (DBCO)Eq TFP Ester-antibodydrug conjugation, and protein structural studies. We8 PET4-DBCOoffer a complete portfolio of crosslinkers tailored to a variety5xof applications with homobifunctional crosslinkers having the6 10x20xsame reactive chemistry at both ends and heterobifunctional40xcrosslinkers having different chemistries at each end. Choosing4the right crosslinker depends on the chemical specificity of the reactive ends, reaction conditions, and spacer composition.2Depending on the application, it may be desirable to adjust the degree of protein labeling (Figure 1). 00.5 1.0 5.0Highlights: [lgG] (mg/mL)Wide selectioncrosslinkers can target a wide variety ofFigure 1. The level of DBCO labeling of antibodies or other proteins functional groups for tailored conjugation on proteins (aminescan be controlled by varying the concentration and ratio of protein and sulfhydryls) and bioorthogonal reactivity (Table 2) to TFP Ester-PEG 4 -DBCO using the Thermo Scientific EZ-Link DBCO Protein Labeling Kit. Diverse compositionchoose from multiple spacer arm lengths, hydrophobicity, and cleavabilityMultiple formatsbulk, Thermo Scientific No-Weigh format, and optimized kit packaging availableTable 2. Overview of crosslinker reactivities and examples.Reactivity classTarget functional groupReactive chemical group ExamplesAmine-reactive NH NHS ester NHS, sulfo-NHS2 Imidoester DMS, DMPCarboxyl- and aminereactive COOH Carbodiimide EDC, DCCMaleimide SMCC, sulfo-SMCCSulfhydryl-reactive SH Haloacetyl SIAB, sulfo-SIAB, SIAPiridyl disulfide PDPHAldehyde-reactive CHO HydrazideBMPH, EMCH, PMPHPhotoreactive Random Diazirine SDA, sulfo-SDAAryl azide Sulfo-SANPAHHydroxyl (nonaqueous)-reactive OH Isocyanate PMPIAzide-reactive N Phosphine NHS-azide, NHS-PEG -azide3 DBCO/alkyne nLearn more at thermofisher.com/proteincrosslinking104 Protein bioconjugation, crosslinking, and modification thermofisher.com/proteinbiology'