b'Single-biomarker quantitation of protein targetsPrecoated ELISA kitsOur ELISA kits are developed to meet specifications typically expected by customers, including standard calibration, precision, sensitivity, specificity, recovery, lot-to-lot consistency, linearity, and parallelism (Table 3).Table 3. Rigorous assay verification of ELISA kits helps ensure consistent, reliable results.Specification Description What does it mean for you?StandardCalibrated to NIBSC, if available Allows accurate quantitation and consistent standard of calibration reference across multiple platformsPrecision Average inter-assay CV 10%; average intra-assay CV 10% Consistent resultsSensitivity Relevant levels of protein are detected for specific assay type Enables detection of low levels of proteinSpecificity Cross-reactivity tests are performed with similar analytes Helps to ensure accurate measurement of the protein of interestRecovery Buffers are optimized to minimize matrix effects Helps to ensure accurate quantitation of samples within a complex matrix, including serum and plasmaLot-to-lotIn-house controls are tested to measure within set ranges Helps to ensure consistent results with new lotsconsistencyLinearity ofLinear results over the quantitative range of the assay Serial dilution of samples are quantitated accuratelydilutionParallelism Recombinant protein standards mimic native proteins Samples can be measured with confidenceCoated ELISA kits that include ready-to-use reagentsQuantitate with confidenceCoated ELISA kits for the quantitation of individual analytes from a variety2.2of sample types provide lower inter- and intra-assay variability. Developed2.0and manufactured for labs that require verified and comprehensively tested1.8Absorption (450 nm)kits, coated ELISA kits offer lot-to-lot consistency and help ensure the1.6highest possible ELISA performance (see an example standard curve in1.4Figure 1). Ready-to-use reagents, including precoated plates, help ensure1.2consistent data throughout your research. 1.00.8Highlights:0.6Robustlow inter- and intra-assay coefficient of variation (CV)0.4Easy to useincludes all required assay buffers and reagents, and a0.2straightforward protocol 010 100 1,000Highly verifiedoptimized for serum, plasma, and cellConcentration (ng/mL)culture supernatantsFigure 1. Standard curve for human IgG1 ELISA.Request bulk packaging or lot reservation, or go custom at thermofisher.com/IASpecialServices76 Immunoassays thermofisher.com/proteinbiology'