b'Protein extraction reagents and kitsGentle formulations designed to maximize protein yield and activityObtain high protein yield from tissues, cells, or subcellular fractions using reagents and kits that are optimized for mammalian, bacterial, yeast, insect (baculovirus), and plant samples (Table 1). These gentle formulations have been verified in multiple tissue types and cell lines, and generally eliminate the need for mechanical cell disruption. These extracts are compatible with a wide range of downstream applications, including protein assays, immunoprecipitation, protein purification, immunoassays, western blotting, gel shift assays, and enzyme assays.Table 1. Overview of sample types and protein extraction reagents and kits.Sample type Goal Recommended Thermo Scientific reagents or kitsPrimary or cultured Total protein M-PER reagentRIPA Lysis and Extraction Buffermammalian cells orextractionT-PER reagentPierce IP Lysis Buffertissues N-PER reagentCultured mammalian Subcellular NE-PER reagentPierce Cell Surface Protein Isolation Kitcells or tissues fractionation or Subcellular Fractionation KitsSyn-PER Reagentorganelle isolation Mitochondria Isolation KitsLysosome Enrichment KitBacterial cellsTotal protein B-PER reagentextractionYeast cellsTotal protein Y-PER reagentextractionInsect cells (baculovirus)Total protein I-PER reagentextractionPlant tissue (leaf, stem, Total protein P-PER reagentroot, flower) extraction14 Protein sample preparation thermofisher.com/proteinbiology'