b'Antibody purificationProteins A, G, A/G, and L have unique properties, which make each one suitable for different types of antibody targets (e.g., antibody subclass or animal species). These and other ligands enable purification of general immunoglobulins from a crude sample (Table 4). Depending on the sample source, an antigen-specific antibody may account for only a small portion of the total immunoglobulin in the sample. For example, generally only 25% of total IgG in mouse serum is specific for the antigen used to immunize the animal.Thermo Scientific POROS MabCapture Select resins demonstrate outstanding binding capacity at a range of flow rates, making them suitable for large-scale bioprocessing needs (Figure 1).Table 4. Antibody purification selection guide for Invitrogen and Thermo Scientific products.High-throughputHigh-throughput Mode Description Recommended product screening batch Batch Pilot ProcessNegativeRemoval of all non- Melon Gel selection immunoglobulin proteinsDynabeads Protein A magnetic beads Pierce High Capacity Protein AMagBeads, alkali stableMabCaptureC High Capacity Protein AResin, alkali stablePierce High Capacity Ni-IMACMagBeads and Resin, EDTA compatibleImmobilizedPierce Protein A Plus Agarose IgGimmunoglobulin-bindingPOROS MabCapture A Select Resin enrichment proteins, to selectivelyDynabeads Protein G magnetic beads remove IgG from aPierce Protein G Plus Agarose serum sample POROS MabCapture G Select Resin Pierce Protein A/G Magnetic Beads Pierce Protein A/G Magnetic Agarose Pierce Protein A/G Plus Agarose POROS MabCapture A/G Select Resin Pierce Protein L Magnetic Beads Pierce Protein L Agarose IgGThiophilic adsorption Pierce Thiophilic Adsorbent enrichmentIgMImmobilized mannan- Pierce Mannan Binding Protein Agarose enrichment binding protein (MBP)IgAImmobilized jacalin, aPierce Jacalin Agarose enrichment D-galactosebinding lectinA 20 20 B 30 30PORPOOSR MOaSb MCapbtCuarepture PORPOOSR MOaSb MCapbtCuareptureIgG bound per mL of resin (mg) IgG bound per mL of resin (mg) IgG bound per mL of resin (mg) IgG bound per mL of resin (mg)G SeGle Scte rlecstin resin A/G AS/eGle Scte rlecstin resinProtePirno Gte iSn eGp hSaerpohsaerose 25 25 PiercPeie Prcroet ePirnotein15 15 4 Fa4st F Falostw F rloeswin resin A/G AP/luGsPalguas raogsaerose20 2010 10 15 1510 105 55 50 0 0 0500500 300300 150150 500500 300300 150150LineLairn eoawrroawter(actme /(chmr)/hr) LineLairn eoawrroawter(actme /(chmr)/hr)Figure 1. Comparison of dynamic binding capacity vs. flow rate. Each column (0.5 cm ID x 5 cm) was packed with 1 mL of resin and was challenged with human IgG (1 mg/mL) at flow rates of 500, 300, or 150 cm/hr (corresponding to residence times of 0.3, 1, and 2 min, respectively). The dynamic binding capacity (total protein loaded) was determined at 10% breakthrough. (A) Comparison between Thermo Scientific POROS MabCapture G Select and Cytiva Protein G Sepharose 4 Fast Flow resins. (B) Comparison between Thermo Scientific POROS MabCapture A/G Select and Thermo Scientific Pierce Protein A/G Plus resins.Protein purification thermofisher.com/proteinbiology 33'