b'HeavyPeptide and LightPeptide AQUA custom standardsImprove quantitative precision for absolute quantitationThe Thermo Scientific HeavyPeptide and LightPeptide AQUA custom synthesis services provide isotopically labeled or unlabeled AQUA (Absolute QUAntitation) peptides with extensive QC for relative and absolute quantitation of proteins. HeavyPeptide and LightPeptide standards are synthesized using the latest Fmoc solid-phase peptide synthesis technology, purified by HPLC, and analyzed by mass spectrometry. Amino acid analysis (AAA) provides the most exact peptide concentration to guarantee the highest level of quantitative precision. The different grades of AQUA peptides are defined by the replicates of AAA, with Basic (1X), QuantPro (2X), and Ultimate (3X).Highlights:Precisepeptide concentration guaranteed from AAASensitiveenables absolute quantitation of low-abundance proteins (fmol)Specific100% peptide sequence specificityFlexiblevariety of purity, modification, and formatting options (Table 2)Table 2. Specifications of HeavyPeptide AQUA-grade standards.AQUA Ultimate Service AQUA QuantPro Service AQUA Basic ServiceResearch stage Validation, ideal for absoluteConfirmation or validation, ideal forDiscovery, confirmation orquantitation biomarker verification validation, relative quantitationAmount/number10 nmol/10 aliquots 10 nmol/10 aliquots 15 to 30 nmolof aliquots 40 nmol/40 aliquots 40 nmol/40 aliquots (0.05 to 0.1 mg)/1 aliquot96 nmol/96 aliquots 96 nmol/96 aliquotsFormulation 5 pmol/L in 5% (v/v) acetonitrile/H O 5 pmol/L in 5% (v/v) acetonitrile/H O Lyophilized2 2Shipment In solution on wet ice In solution on wet ice Dry at room temperatureConcentration 510% 1025% NAprecisionPeptide length* Up to 15 amino acidsPurity 97%Isotopic 99%enrichmentStandard (light) Yespeptides availableDelivery formatGlass vialProduction time 57 weeks standard AQUAQuality control MALDI-TOF MS, HPLC, quantitative AAASingle or double phosphorylation (pY, pT, or pS) Modifications Cysteine carbamidomethylation (CAM) Methionine sulfoxide [Met(O)] Other modifications available on requestAdditional light amino acids to extend peptide length Optional services Additional heavy amino acids within the peptide sequence Multiple solvents, concentrations, and aliquot sizes available* Please inquire about longer peptide lengths.Learn more at thermofisher.com/heavypeptideMass spectrometry thermofisher.com/proteinbiology 93'