b'Bolt Bis-Tris Plus and NuPAGE Bis-Tris gels offer: Preserved protein integrityneutral-pH formulation minimizes protein modifications and degradation (Figure 1)Superior band quality and band volumeBis-Tris gels are designed to deliver sharp, straight bands with higher band volume (Figure 2)High sample volume capacitywedge-well design of Bolt Bis-Tris Plus mini gels allows detection of proteins in very dilute samples or measurement of low-abundance proteinsMore efficient western blot transferneutral pH prevents reoxidation of reduced samples during protein transferHigh lot-to-lot consistencycoefficient of variation (CV) of only 2% for R values f(migration)Long shelf life16 months at room temperatureMagicMark GST IKK EPHB3 HCK MAPK14 FLT1 DDR2 MagicMark IKKstandard standard GST EPHB3 HCK MAPK14 FLT1 DDR2Bolt Bis-Tris Plus gel Bio-Rad TGX Tris-glycine gelFigure 1. Bolt Bis-Tris Plus mini gels help provide better westernNuPAGE Bis-Tris 412% gradient midi gelblotting results. A western blot of a Bolt gel shows clean, sharp protein signals corresponding to only full-length proteins, whereas aFigure 2. Publication-quality protein electrophoresis gel western blot of a Bio-Rad TGX gel shows multiple low molecularresults using a NuPAGE Bis-Tris 412% gradient midi gel and weight degradation products. Protein kinases implicated in cancerthe SureLock Tandem Midi Gel Tank. Lanes 1, 20: 5 L Thermo (IKK, EPHB3, HCK, MAPK14, FLT1, and DDR2) were analyzed on aScientific PageRuler Broad Range Unstained Protein Ladder; lanes Bolt Bis-Tris Plus gel and a Bio-Rad TGX Tris-glycine gel. The purified27: 10 g, 8 g, 6 g, 4 g, 2 g, 1 g of HeLa lysate; lanes 8, 13: kinases (50 ng each), along with Invitrogen MagicMark XP Western5 L Invitrogen Novex Mark12 Unstained Standard; lanes 912: Protein Standard and purified recombinant GST protein, were loaded on240 ng, 180 ng, 120 ng, 60 ng of protein mix containing -galactosidase, a 10-well, 412% Bolt gel and a 10-well, 420% Bio-Rad TGX gel. Thelactate dehydrogenase, and lysozyme; lanes 1419: 10 g, 8 g, samples were separated and transferred to 0.45 m PVDF membranes6 g, 4 g, 2 g, 1 g of E. coli lysate. The gel was run in Invitrogen using the respective manufacturers protocols. Immunodetection wasNuPAGE MOPS running buffer, and stained with Invitrogen performed using an anti-GST antibody and Invitrogen WesternBreezeSimplyBlue SafeStain.chemiluminescence detection. The blots were imaged using an LAS-1000 system (FujiFilm).Find out more at thermofisher.com/nupage46 Protein gel electrophoresis thermofisher.com/proteinbiology'