b'Table 1. Characteristics of our immunoassay kits.Type of immunoassayBuild-it-yourselfUncoated ELISAs andCoated Instant ProQuantum ProcartaPlex immunoassays antibody pair kits ELISA kits* ELISA kits HS kits immunoassays**Ready-to-useNo; need overnightYes; need overnightYes Yes Yes Yesreagents coating process coating processAnalytical 10 pg/mL 10 pg/mL 10 pg/mL 5 pg/mL 0.1 pg/mL 10 pg/mLsensitivityDynamic range5250 pg/mL 5500 pg/mL 5250 pg/mL 7.8500 pg/mL 0.0645,00052,000 pg/mLpg/mLSample volume 10100 L 10100 L 10100 L 2550 L 25 L 2550 LIncubation time4 hr 34.5 hr 2.54 hr 4 hr 2 hr 3.5 hrAbility to multiplex No No No No No YesNumber of targets1 1 1 1 1 180measured per wellReadout HRP-TMBHRP-TMB (colorimetric) HRP-TMBHRP-TMBFAM RPE(colorimetric) (colorimetric) (colorimetric) (fluorescent) (fluorescent)InstrumentationMicroplate reader Microplate reader Microplate reader Microplate reader RT-PCR Luminex needed instrumentInstrument 2 min 2 min 2 min 2 min 3672 min 2060 minread time * Values in this table refer to standard colorimetric kits. Ultrasensitive kits are also available.** Immunoassays for the Luminex platform. Every assay has its own specifications. Please consult the protocol for your specific immunoassays or kits.Table 2. List of components for different immunoassay kits.Type of immunoassayImmunoassayAntibody pairCoated Instant ProcartaPlex assays components ELISA kits ELISA kits ELISA kits ProQuantum HS kits (all platforms)96-well plate Optional NA Flat bottomPretitratedPrecoated PrecoatedPrecoatedcapture antibody capture beadPretitrated detection antibodyRecombinant Additionalprotein standard well strips includedStop solution Not included NA NASubstrate solutionNA SA-PEAssay buffer Sample diluentWash buffer Not included NA Coating bufferNA NA NA NANote: Our immunoassays are available in various kit sizes. Kits are calibrated to National Institute for Biological Standards and Control (NIBSC) or World Health Organization (WHO) standards (if available). SA = streptavidin, PE = phycoerythrin, NA = not applicable.Go to thermofisher.com/immunoassays to find:Our helpful immunoassay selectionDetailed information on all of our antibodyImportant data demonstrating our guide that allows you to search forpair kits, ELISA kits, and ProQuantumassay specificity and sensitivityassays based on your target protein high-sensitivity and multiplex assay kitsImmunoassays thermofisher.com/proteinbiology 75'