b'Protein sample preparationValidated reagents for robust and reproducibleHigh-Select Abundant Protein Depletion Reagents and sample processing Phosphopeptide Enrichment KitsThermo Scientific reagents and kits are optimized forThe High-Select product line offers kits and resins for mass spectrometry sample preparation for every step of thebetter detection of low-abundance proteins through sample process, from depletion strategies to reduce complexity, todepletion and enrichment. Thermo Scientific High-Select protein extraction, enrichment, and cleanup for the isolation ofHSA/Immunoglobulin and Top14 Abundant Protein Depletion low-abundance proteins. Post-digestion, peptide enrichment,Resins deplete the abundant protein and antibody components fractionation, and cleanup steps are often critical for properof human plasma samples, helping to prepare the samples analysis. The Thermo Scientific Pierce Quantitative Colorimetricfor mass spectrometry or 1D or 2D electrophoresis (Table 3). and Fluorometric Peptide Assays enable sample normalizationThermo Scientific High-Select Phosphopeptide Enrichment following digestion. Kits and Resins available in agarose and magnetic bead formats Highlights: enable efficient and selective enrichment of phosphorylated peptides for mass spectrometry analysis. Comprehensivereagents and kits for every step of the sample preparation workflow, both before and afterHighlights:protein digestion Optimizedprotocols and reagents designed to maximize Optimizedreagents and kits are designed to maximize yieldresults in a fraction of the processing timewithout sacrificing quality High qualitygreater than 95% depletion of the top Versatileproducts available as complete kits or asserum/plasma proteins and phosphopeptide recovery with stand-alone reagents, featuring the Thermo Scientific90% selectivity (Figures 68)EasyPep and High-Select lines of sample prep kits Flexiblekits and resins provided in several formats to Compatiblekits are designed to be compatible with massaddress sample input and throughputspectrometry analysis Scalablecompatible with automationValidatedproducts have been fully tested, and processed samples have been analyzed using Thermo Scientific mass spectrometers Table 3. Proteins removed by High-Select abundant protein depletion spin columns. HSA/immunoglobulin Top14 columnsAlbumin Albumin -Acid glycoproteinIgG IgG FibrinogenIgA IgA HaptoglobinIgM IgM 1-AntitrypsinIgD IgD 2-MacroglobulinIgE IgE TransferrinIgG (light chains) IgG (light chains) Apolipoprotein A-194 Mass spectrometry thermofisher.com/proteinbiology'